PHARMACOKINETIC STUDIES ON THE HEPATOTOXICITY OF LUTEOSKYRIN (II) EXTRACTION AND IDENTIFICATION OF 3H-LUTEOSKYRIN FROM THE LIVER AND EXCRETA IN MICE

[摘要] References(8)Cited-By(3)3H-luteoskyrin was injected subcutaneously into male and female mice and its radioactive compounds in the liver and excreta were analyzed. The radioactive compounds in the subcellular fractions of the liver could be extracted into ether under acidic conditions, and those in the excreta were recovered by extraction with ethanol. One of the radioactive compounds isolated from the liver and the feces was identified as unmetabolized 3H-luteoskyrin by thin-layer chromatography, gel filtration and spectrophotometry. In the liver subcellular fractions, mitochondria and cell debris contained 3H-luteoskyrin almost exclusively, while in the postmitochondrial fraction 3H-luteoskyrin corresponded to one half of the total radioactive compounds. The urine contained radioactive compounds other than 3H-luteoskyrin one of which was separated by the gel filtration. It was concluded that, after preferential distribution in the subcellular fractions, especially in the mitochondria and cell debris, of the liver, luteoskyrin is excreted as such in the feces and as a metabolite in the urine.

[学科分类] 药理学