Perivascular Purinergic Nerve-Induced Vasoconstrictions in Canine Isolated Splenic Arteries

[摘要] References(12)Cited-By(6)We tried to induce selective perivascular purinergic nerve stimulation in isolated canine splenic arterial preparations, using the cannula insertion method.Under the conditions of periarterial electrical stimulation(ES), i.e., trains of 1, 3 and 10 pulses, 1−ms pulse duration and 10−V amplitude at 1 Hz, monophasic vasoconstriction was consistently induced.The ES−induced vasoconstriction was not influenced by prazosin in doses that completely inhibited noradrenaline−induced vasoconstrictions, but it was suppressed by α, β−methylene ATP, a P2X purinoceptor desensitizer.Thus, it is indicated that a selective purinergic transmitter release is readily obtained in the isolated splenic arterial preparation.

[学科分类] 药理学

[关键词] Dog splenic artery;Perivascular electric stimulation;P2X Purinoceptor