Captopril Increases the Affinity of Bradykinin Receptor Binding Sites in Bovine Coronary Arterial Endothelial Cells

[摘要] References(15)Cited-By(2)In a radioligand binding study using bovine coronary artery endothelial cell membranes, captopril changed a single bradykinin(BK)binding site(Kd=1.77 nM, Bmax=60.2 fmol/mg protein)to high−(Kd=0.68 pM, Bmax=17.7 fmol/mg protein)and low−(Kd1.00 nM, Bmax=72.5 fmol/mg protein)affinity binding sites.This effect was reversed by GppNHp.Captopril also enhanced BK−induced endothelium−dependent relaxation in saponin−treated coronary rings, and GppNHp partially suppressed this enhancement.These results suggest that captopril may affect BK receptors that couple to G−proteins.

[学科分类] 药理学

[关键词] Bradykinin;Captopril;Coronary artery