Enhancement of [3H]Clonidine Binding to Rat Cerebral Synaptic Membranes by Treatment with Arachidonic Acid, Prostaglandin (PG) D2, PGE2 and PGF2α

[摘要] References(12)Cited-By(2)Pretreatment of cerebral synaptic membranes with arachidonic acid (10 nM—1 μM) at 37°C for 40 min caused a significant increase in [3H]clonidine binding but pretreatment at 4°C did not. Scatchard analysis showed that 100 nM arachidonic acid pretreatment resulted in a significant elevation of the Bmax value of the low affinity site. PGD2, PGE2 and PGF2α (10-100 μM) pretreatment also enhanced binding. These polyunsaturated fatty acids could be involved in regulation of the α2-adrenoceptor binding in rat cerebral cortical membranes.

[学科分类] 药理学