Further Studies on the Endogenous Serotonin-Uptake-Inhibitor-Like Substances in the Human Cerebrospinal Fluid

[摘要] References(31)Cited-By(1)The properties of endogenous substances that inhibit 5HT uptake in human cerebrospinal fluid (CSF)were investigated. Human CSF was loaded onto a Sephadex G-25 column, and each fraction was tested for its ability to inhibit [3H]paroxetine binding in monkey brain preparations. We found four different inhibitory peaks with respective molecular weights (M.W.)of > 12400, 2000 and two of 3H]paroxetine binding noncompetitively (decreased Bmax and did not change Kd)and inhibited [3H]5HT uptake noncompetitively (decreased Vmax and did not change Km), but had no effect on either [3H]norepinephrine uptake or [3H]dopamine uptake. These results suggest that the endogenous substances that selectively inhibit 5HT uptake are present in human CSF as low molecular weight compounds.

[学科分类] 药理学

[关键词] Serotonin uptake;Paroxetine binding;Cerebrospinal fluid (human);Brain (monkey);Antidepressant (endogenous)