The Cytosolic Acidification in Rat Parotid Cells Is Associated with an Increase in Cytosolic Ca2+ Concentration

[摘要] References(15)Cited-By(1)A transient decrease in cytosolic pH ([pH]i)in rat parotid cells was evoked by the addition of carbachol (CCh), phenylephrine, or substance P, whereas isoproterenol and dibutyryl cyclic AMP had little or no effect on [pH]i. The decrease in [pH]i induced by the Ca2+-mobilizing agonists was also observed in Ca2+-free medium, but not when the intracellular Ca2+ stores were previously depleted. lonomycin and thapsigargin elicited a decrease in [pH]i with an increase in cytosolic Ca2+ concentration ([Ca2+]i). The protein kinase C activator and inhibitor had no effect on the agonist-induced decrease in [pH]i. These results suggest that the cytosolic acidification is associated with an increase in [Ca2+]i.

[学科分类] 药理学

[关键词] pH (cytosolic);Ca2+ concentration (cytosolic);Parotid cell (rat)