Calcium-Induced Vasodilation Due to Increase in Nitric Oxide Formation in the Vascular Bed of Rabbit Ear Preparation

[摘要] References(23)Cited-By(4)Participation of calcium-induced vasodilation (due to an increase in synthesized nitric oxide (NO)content in endothelial cells)in the arterio-venous circulation, including the vascular bed was investigated by the vessel perfusion method in the isolated rabbit ear preparation. The perfusion medium used was a tris-buffered solution. When CaCl2 (6.25, 12.5 and 25 mg)was injected in the perfused vessel of the rabbit ear preparation, dose-dependent vasocontraction was observed when vascular tone was kept at a normal level. However, CaCl2 dose-dependently induced vasodilation of the vessel when it was continuously contracted by norepinephrine (1.2 × 10-7 M). This calcium-induced vasodilation was inhibited in the presence of NG-nitro-L-arginine (5 × 10-5M), a selective inhibitor of NO synthesis, and methylene blue, a guanylate cyclase inhibitor, although it was rarely affected by indomethacin (10-5 M), a cyclooxygenase inhibitor. Calcium-induced vasodilation was also obtained in the in situ circulation containing vascular bed, and this suggests that the vasodilation was due to a Ca2+-induced increase in the synthesis of NO derived from endothelial cells.

[学科分类] 药理学

[关键词] Vasodilation (Ca2+ -induced);Nitric oxide;NG -Nitro-L-arginine;Vascular bed;Ear (rabbit)