In Vitro Oxygenation Injury to Slices Prepared from Ischemic Kidney in Rats

[摘要] References(15)Cited-By(1)When cortical slices prepared from rat kidneys made ischemic were incubated under a 100% oxygen atmosphere, lipid peroxidation increased and the ATP level decreased. Such oxygenation of the slices was accompanied by decreases in gluconeogenesis and the glutathione level, but an anti-oxidant, N, N''-diphenyl-p-phenylenediamine, prevented the increase in lipid peroxidation without affecting decreases in ATP and glutathione levels, and gluconeogenesis. The results suggest that postischemic oxygenation of slices generates free radicals that cause the production of lipid peroxidation not associated with tissue injury.

[学科分类] 药理学

[关键词] Ischemic kidney;Lipid peroxidation;Oxidative injury