Basic Fibroblast Growth Factor Ameliorates Rotational Behavior of Substantia Nigral-Transplanted Rats with Lesions of the Dopaminergic Nigrostriatal Neurons

[摘要] References(35)Cited-By(8)Basic fibroblast growth factor (bFGF) was injected with dissociated substantia nigral cells into the striatum of rats prepared by unilateral lesion of the nigrostriatal pathway with 6-hydroxydopamine. The transplanted cells with 5 and 50 ng bFGF reduced the apomorphine-induced rotations by 40 and 30%, respectively, while the decrement of rotations was only 15% in the grafted control without bFGF. Immunohistochemical staining with anti-tyrosine hydroxylase antibody showed that bFGF also tended to increase the number of grafted catecholaminergic neurons along the tracts. In the case of 50 ng bFGF treatment but not 0 or 5 ng bFGF treatment, however, severe gliosis was detected along the grafted region by staining of anti-glial fibrillary acidic protein antibody. These immunohistochemical studies suggested that high-dose bFGF induced extensive gliosis, which might affect the survival of the grafted neurons.

[学科分类] 药理学

[关键词] Basic fibroblast growth factor (bFGF);Cell transplantation;Rotational behavior;Apomorphine