EFFECT OF PHOSPHOLIPASE A, C, AND D ON SUGAR AND AMINO ACID TRANSPORT IN RAT UTERUS

[摘要] References(22)Cited-By(5)It is widely known that estrogen can stimulate transport of both sugars and amino acids into rat uterus (1-6). This estrogen effect on transport may reflect the anabolic activity of the hormone in uterus but its mechanism is little known. Phospholipid in tissues is an essential component of cell membrane which is composed mainly of lipid and protein, though the basic arrangements of these two components are not clear. On the other hand, phospholipid in rat uterus is the major organic component that undergoes rapid acceleration of metabolism and increases at an early phase after estrogen administration (7). It can be presumed that cell membrane in an organ plays an important role in the transport of substrates. Recently, the role of phospholipid on sugar transport was reported and it was observed that the rate of intracellular 3-0-methylglucose space in the castrated rat uterus increased by the addition of phospholipid (8). On the basis of these results, it seemed of interest to study the role of phospholipid on sugar and amino acid transport in rat uterine cell membrane, and such an examination was carried out by using phospholipase A (phosphatide acyl-hydrolase, EC 3.1.1.4), phospholipase C (phosphatidylcholine-phosphohydrolase, EC 3.1.4.3), and phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4). This report shows that the phosphate position and/or part of basic groups of phospholipid in a membrane tissue may be playing a significant role on sugar and amino acid transport in rat uterus that was increased by estrogen.

[学科分类] 药理学