STUDIES ON THE FUNCTION OF CELL MEMBRANE

[摘要] References(16)Cited-By(11)Purity of the cell membrane fraction from CCl4-treated rats was studied using markers of other fractions; DT-diaphorase in supernatant, cytochrome c oxidase and succinate-cytochrome c reductase in mitochondria, and NADPH-cytochrome c reductase, glucose-6-phosphatase and RNA in microsomes. Cell membrane fractions were not contaminated with supernatant DT-diaphorase, but were contaminated by mitochondrial fraction in both normal and CCl4-treated rats to some extent. Glucose-6-phosphatase activity and RNA content of the normal membrane fraction were 9 % and 6 % of microsomes, respectively. Administration of CCl4 did not increase the contamination. However, NADPH-cytochrome c reductase activity was increased about 4 times as was the case with NADH-cytochrome c reductase following CCl4 treatment. Thus, it may be concluded that elevation of NADH-cytochrome c reductase activity in the cell membrane fraction 6 hr after administration of CCl4 does not result only from the contamination with other subcellular fractions during the isolation procedure.

[学科分类] 药理学