GTPγS-Induced Ca2+ Activated Cl− Currents: Its Stable Induction by Gqα Overexpression in Xenopus Oocytes

[摘要] References(9)Cited-By(3)In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPγS) (30 mM, 5 nl) did not induce Cl− current in 11 out of 22 oocytes. Injection of increased concentration of GTPγS (100 mM, 5 nl) into the oocytes induced Cl− currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gqα, GTPγS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl− currents. These results indicate that heterogeneous expression of Gqα in Xenopus oocytes provides a useful system for studying the functional roles of Gqα in regulating cellular events.

[学科分类] 药理学

[关键词] Gq;GTPγS;oocyte