Effects of Pollen-Extract Components, Diamines and Derivatives of Feruloylputrescine on Isolated Bladder and Urethral Smooth Muscles of Mice

[摘要] References(19)The contracting or inhibitory effects of pollen-extract components, diamines and derivatives of feruloylputrescine (FP) were investigated on the isolated bladder or urethral smooth muscles of mice. Among the nine diamines (NH2•(CH2)n·NH2, n=2-10) tested, five of them with shorter carbon chains (n=2-6) (0.1-30.0 mM) only slightly contracted the bladder strips and to some extent inhibited the noradrenaline (NA, 1.77 μM)-induced contraction of urethral strips. 1, 5-Diaminopentane (C5), a component of the pollen-extract, inhibited most effectively the NA-induced contraction of urethral strips with an IC50 value of 2.3 mM (95% confidence limit: 2.0-2.6 mM). FP, also a component of the pollenextract, inhibited the NA-induced contraction of urethral strips in a non-competitive manner, producing 32.5±5.5% (N=5) inhibition at 378 μM. Among the derivatives of FP, feruloylcadaverine inhibited urethral contraction most potently, producing 46.3±7.1% (N=5) inhibition at 359 μM. These derivatives had no effect on bladder contraction. In contrast, four diamines with longer carbon chains (n=7-10) contracted the bladder strips (3-30 mM) and potentiated the NA-induced contraction of urethral strips (10 μM-3 mM). Thus, the components of the pollenextract, FP and C5, potently inhibited urethral contraction, which may facilitate the discharge of urine in vivo.

[学科分类] 药理学