A P Element Excision-Derived Mutation in Drosophila melanogaster

[摘要] The purpose of the work described in this Thesis was to isolate and mutate novel genes essential in the development of the Drosophila melanogaster nervous system. Two marked P element enhancer trap strains were found to express the reporter gene lacZ in spatially and temporally regulated patterns in the nervous system. Excisions of the P element construct in one of these strains, A22, were detected in approximately 68% of the A22-derived progeny which were recognised by the loss of the rosy gene. 19 strains were subsequently found to have a recessive mutant phenotype believed to be a result of imprecise excision of the P element. This represents a mutation rate of approximately 24%. Each of these 19 strains had a recessive mutation that resulted in abnormal wing development. Other mutant phenotypes were not detected in any of the excision-derived strains. No similar mutations are known to be located at the site of P element insertion of the ancestral A22 strain and therefore these excision-derived mutants are considered novel. Four excision-derived mutants were selected for further characterisation and these were found to be cold-sensitive. The severity and frequency of the mutant phenotype was increased when these four strains were raised at 20

[发布机构] University:University of Glasgow

[关键词] Genetics