Validation of method for simultaneous determination of four organic acids in frozen fruit pulp by high performance liquid chromatography

[摘要] Validation of a rapid method for the determination of ascorbic, citric, fumaric and tartaric acids in stored pulp fruit and its application as a quality parameter was performed. The validation parameters showed that for the four evaluated acids, the method presented low limits of detection (LOD) and quantification (LOQ), indicating good precision and accuracy, thus representing an important tool for quality assessment of stored fruit pulp. The results showed that the concentration of organic acids generally decreased with longer storage time in the fruit pulp under study. Amongst all the organic acids under investigation, ascorbic proved the least stable.

[学科分类] 化学（综合）

[关键词] HPLC-UV;stored fruit pulp;organic acids.