Immobilized metal-ion affinity chromatography (IMAC) of biomolecules: fundamental aspects and technological applications

[摘要] Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.

[学科分类] 化学（综合）

[关键词] immobilized metal-ion affinity chromatography;purification;biomolecules.