Problem statement: Shigellosis is a common infectious disease especially inunderdeveloped countries. The bacteria are primarily transmitted through the faecal-oral route. Theinflammatory process of acute Shigella infection affects the colon and is characterized clinically byfever, cramping abdominal pain with frequent loose stools that might contain mucus, pus and blood.Approach: This study determined the susceptibility of Shigella isolates to antibiotics. Afterward,plasmid isolation from pathogenic Shigella will carried out to achieved information concerning to thepresence of plasmid DNA in Shigella isolates. Plasmid curing will be done to seek correlation betweenresistance to antibiotics and plasmid occurrence in Shigella. Results: We found that the incidence ofdiarrhea in male is almost similar to female. The distribution of Shigella spp., in male more than infemale represented 54.54 and 45.46% respectively. Conclusion: Approximately 89.5% of the diarrheacases had no bacterial pathogen, suggested of probability of viral infection.
