Problem statement: Condoms are widely used birth control measures which are made of natural latex or synthetic materials like polyurethane or polyisoprene as matrix with lubricants added to make them ready to use commodities. Absence of sterilization method along with presence of lubricant like petroleum jelly (that are applied on the outer surface), prompted the investigators to look for microbes from the surface of male condoms. Approach: The culture based method was used for the study. This study reports the isolation and preliminary characterization of microbes from unused new condoms of ten different brands available in the chemist