Strains of Klebsiella Pneumoniae from Intensive Care Unit Producing CTX-M-15 Extended Spectrum Beta Lactamases | Science Publications

[摘要] Problem statement: Resistance to third generation cephalosporins due to acquisition and expression of Extended Spectrum β-Lactamase (ESBL) among Gram-negative bacteria is on the increase. Infections involving extended spectrum beta lactamase bacteria are associated with significant morbidity and mortality. Therefore, infections due to ESBL isolates continue to pose a serious challenge to infection management worldwide. Since screening for ESBL is not a common practice in hospitals in Enugu state, this study was undertaken to characterize ESBL genes in K. pneumoniae strains from intensive care unit of the University of Nigeria Teaching Hospital (UNTH) Enugu. Approach: Over a period of 29 months, 57 patients out of 140 receiving treatment in the intensive care unit of the University of Nigeria Teaching Hospitals Enugu were found to be infected with extended-spectrum β-lactamase-producing strains of K. pneumoniae. Species identification of K. pneumoniae strains was performed by Standard Microbiology methods and re-confirmed by MALDI-TOF technology. Phenotypic characterization of Extended Spectrum Beta Lactamase (ESBL) was determined by double disc synergy test and presence of ESBL genes was determined by specific PCR. Results: All ESBL producers were positive in a PCR for blaCTX-M-1 cluster and on sequencing, blaCTX-M-15 were found to be present. Genotypic characterization of extended spectrum beta-lactamase producing K. pneumoniae showed that all isolates carried CTX-M-15and SHV genes, 41(71.9%) carried aac (6

[学科分类] 微生物学和免疫学

[关键词] Extended Spectrum B-Lactamase (ESBL);University of Nigeria Teaching Hospital (UNTH);Klebsiella pneumoniae;CTX-M-15;Intensive care unit