In vitro fibroblast growth stimulatory and in vivo wound healing activity of Cleome viscosa

[摘要] Cleome viscosa L. (Cleomaceae) is known as Asian spider flower distributed throughout the greater part of India and widely used traditional medicine of Indian-Ayurvedic and Chinese-medicine system. In the present study, petroleum ether, chloroform, methanol and water successive extracts of C. viscosa leaves were evaluated for in vitro antioxidant, antimicrobial and in vitro fibroblast proliferation. In vivo wound healing activity performed on mice excision model using gentamicin sulfate (0.01 % w/w) hydrogel as reference standard. Petroleum ether (CvPE) and chloroform (CvCE) extracts of C. viscosa were found inactive in antioxidant evaluations, whereas methanol extract (CvME) showed potent DPPH (IC₅₀-56.39 μg/ml) and superoxide scavenging activity (IC₅₀-919.70 μg/ml) with highest gallic acid equivalency, among all other extracts. CvME showed with 0.5 mg/ml MIC against B. subtilis and S. aureus. In vitro evaluation for fibroblast proliferation showed that CvPE (59.73 %, 200 μg/ml) and CvCE (71.95 %, 200 μg/ml) decreased the cell viability as compared to normal control, whereas CvME (119.72 %, 50 μg/ml) and water (CvWE) extract (103.80 %, 25 μg/ml) induced the fibroblast proliferation. In vivo wound healing experiment showed that topical application of CvME (2.5 % w/w) and gentamicin sulfate hydrogel significantly increased the wound contraction (75.30 % and 78.74 %), respectively as compared to vehicle control group on 12th post-operative day. Histopathological evaluation also suggested the increased infiltration of fibroblast and abundant hair follicle growth in CvME and gentamicin sulfate and supported the in vitro findings of fibroblast proliferation. Thus the above results justify the rationale of the traditional use of C. viscosa as a wound healing medicine.

[学科分类] 药学

[关键词] Wound healing