Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq

[摘要] Free radical oxygen species cause protein carbonylation, an irreversible oxidative damage associated with diseases such as liver injury, diabetes mellitus, arthritis, cancer and Alzheimer. To determine the inhibitory activity of the protein carbonylation and the hepatoprotective effect of ethanol extract of the fruits of Caesalpinia coriaria Jacq (Fabaceae). Scavenging activity of 2,2-diphenyl-1- picrylhydrazyl radical (DPPH^•) and the radical cation 2,2’-azino-bis(3-ethylbenzothiazoline-sulfonic acid) (ABTS^•+) of the extract of C. coriaria at 500 mg / L, was evaluated in a preliminary test. Inhibitory activity of the protein carbonylation isolated from mouse liver using Western methodology with dinitrophenylhydrazine (DNPH) probes was evaluated. The hepatoprotective activity of the extract was evaluated in vivo in Wistar rats using carbon tetrachloride (CCl₄) as toxic orally. The degree of hepatoprotection was determined by measuring the serum liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and by histopathological analysis of rat liver. The rate of capture of the radicals DPPH^• and ABTS^•+ by the extract of C. coriaria was higher than 90 % in both cases. The extract also showed a better inhibitory effect on the carbonylation of the proteins than butyl hydroxytoluene (BHT) used as positive control. The extract of C. coriaria produced a significant reduction of AST concentration (from 1667,67 ± 394,27 to 718,00 ± 24,85 U/L ) and ALT (from 967,67 ± 118,30 to 625.67 ± 60,98 U/L), indicating liver cell damage attenuation. The extract of C. coriaria produced an important antioxidant activity and moderate hepatoprotective effect against CCl₄-induced toxicity.

[学科分类] 药学

[关键词] Alanine aminotransferase