Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence

[摘要] The fluorescent neutral lipid stain, nile red, was used to examine cell-specific neutral lipid levels in natural assemblages of Antarctic sea ice microalgae. Neutral lipid:chlorophyll, neutral lipid:particulate carbon (PC) and neutral lipid:particulate nitrogen (PN) ratios were highest in communities dominated by Nitzschia spp. and Navicula glaciei van Heurck. The lowest specific neutral lipid content was estimated in the congelation ice samples dominated by the diatom Amphiprora spp., and in surface assemblages dominated by Phaeocystis pouchetii Hariot and the dinoflagellate Gymnodinium sp. Scatter plots of neutral lipid on PC and PN, which included data from all assemblages, showed that assemblages dominated by P. pouchetii and Amphiprora spp. clustered near the origin reflecting their relatively lower specific neutral lipid levels, compared with assemblages dominated by N. glaciei and Nitzschia spp. Cellular PC:PN was significantly (P

[学科分类] 自然科学（综合）