Diverse NF-κB expression in epiretinal membranes afterhuman diabetic retinopathy and proliferative vitreoretinopathy

[摘要] Purpose: Formation of epiretinal membranes (ERMs) afterproliferative diabetic retinopathy (PDR) and proliferativevitreoretinopathy (PVR) results in progressive deterioration of vision,but its pathogenic mechanisms are still unknown. This study wasconducted to examine the role of nuclear factor kappa B (NF-κB) inthe formation of ERMs after PDR and PVR.Methods: ERM samples were obtained by vitrectomy from 10 patientswith PDR (aged 53±12 years with 14±5 years of diabetes), 20patients with PVR, and 17 patients with idiopathic ERMs. Ten PVR and 17idiopathic ERM samples were processed for reversetranscription-polymerase chain reaction (RT-PCR) analysis. In addition,10 PDR and 10 PVR membranes were processed for immunohistochemicalanalysis.Results: NF-κB mRNA expression levels were significantlyhigher (10 of 10 versus 9 of 17 subjects in idiopathic ERM, p=0.0119) inPVR subjects. Immunohistochemical analysis showed NF-κB proteinexpression in 8 of the 10 PDR samples as well as all 10 PVR samples, andNF-κB positive cells were partially double labeled with glial cellmarkers. Interestingly, NF-κB protein was also overlapped withangiogenic factor interleukin-8 (IL-8) in glial cells as well asvascular endothelial cells.Conclusions: These results suggest that NF-κB is involved inthe formation of both glial and vascular endothelial cell components,and that these two cell types might have functional interactions thatlead to the enlargement of intraocular proliferative membranes.

[学科分类] 生物化学/生物物理