Laser scanning analysis of cold cataract in young and old bovinelenses

[摘要] Purpose: This research involves the use of a laser scanninginstrument to evaluate the formation of cold cataracts in young andolder bovine lenses.Methods: Bovine lenses from 18 (n=14) week old calf eyes andapproximately 10 (n=7) year old animals were extracted in a sterileenvironment. The lenses were placed in a specialized glass chamber withtemperature controlled circulating culture medium. The temperature cycleinside the chamber started at 37 °C, slowly cooled to 4 °C, andthen warmed back up to 37 °C. A laser scanning system (ScanToxTM)was used to analyze the optical quality of bovine lenses during acooling and warming cycle.Results: The relative light transmittance was measured as a functionof pixel excitation caused by refracted beams and compared topre-treatment measures. Each lens from each age group showed asignificant decrease in transmittance at 4 °C, which recovered whenthe lenses were warmed to 37 °C. Lenses from young eyes showed lessloss of refracted beam intensity than lenses from older eyes (32% versus34%), although the difference was not significant.Conclusions: The results of the present study indicate coldcataracts can be induced in both old and young bovine lenses, as shownby using a scanning laser instrument.

[学科分类] 生物化学/生物物理