The role of macrophage migration inhibitory factor in ocular surface disease pathogenesis after chemical burn in the murine eye

[摘要] Purpose: To evaluate the role ofmacrophage migration inhibitory factor (MIF) in the wound healingprocess following severe chemical burns to the ocular surface. Methods: Chemical burning of the ocularsurface was induced in mice (C57BL/6) via the application of 0.1 MNaOH. Macrophage migration inhibitory factor (MIF), tumornecrosis factor-α (TNF-α), and interleukin-1β (IL-1β)mRNAexpression in the ocular surface and lacrimal gland was evaluated viareal-time reverse transcription PCR on days 2, 7, and 30 afterinduction of the chemical burn. The expression of MIF protein in theocular surface and lacrimal gland was evaluated via western blotanalysis. Immunohistochemical staining was conducted to detect MIF andvasculoendothelial growth factor in the cornea during the wound healingprocess. The angiogenic role of MIF was further evaluated using an 8–0polyglactin suture technique to induce corneal neovascularization. Results: MIF, TNF-α, and IL-1βmRNA expression were elevated significantly in the ocular surface up today 30 after chemical burn induction. TNF-α alone was elevatedin the lacrimal gland. MIF protein elevation was confirmed via westernblot analysis, and the spatial similarity of MIF and VEGF expression inthe cornea was noted during the wound healing process. 8–0 polyglactinsutures soaked in MIF induced significantly higher numbers of newvessels on the mouse cornea after 7 days (p=0.003, Mann–Whitney test). Conclusions: These findings indicatethat MIF performs a crucial role in wound healing on the ocular surfaceafter the induction of chemical burns.

[学科分类] 生物化学/生物物理