Evaluation of monkey intraocular pressure by rebound tonometer

[摘要] Purpose: To evaluate the usefulness ofthe TonoVet™ rebound tonometer in measuring intraocular pressure (IOP)of monkeys. Methods: The accuracy of the TonoVet™rebound tonometer was determined in cannulated eyes of anesthetizedrhesus monkeys where IOP was controlled by adjusting the height of aconnected perfusate reservoir. To assess the applicability of theequipment through in vivo studies, the diurnal fluctuation of IOP andeffects of IOP-lowering compounds were evaluated in monkeys. Results: IOP readings generated by theTonoVet™ tonometer correlated very well with the actual pressure in thecannulated monkey eye. The linear correlation had a slope of0.922±0.014 (mean±SEM, n=4), a y-intercept of 3.04±0.61, and acorrelation coefficient of r2=0.97. Using this method,diurnal IOP fluctuation of the rhesus monkey was demonstrated. Thetonometer was also able to detect IOP changes induced bypharmacologically active compounds. A single topical ocularinstillation (15 μg) of the rho kinase inhibitor, H1152, produced a5–6 mmHg reduction (p<0.001) in IOP, lasting at least 4 h. Inaddition, topical administration of Travatan®, a prostaglandin agonist,induced a small transient IOP increase (1.1 mmHg versus vehiclecontrol; p=0.26) at 2 h after treatment followed by a pressurereduction at 23 h (−2.4 mmHg; p<0.05). Multiple daily dosingwith the drug produced a persistent IOP-lowering effect. Threeconsecutive days of Travatan treatment produced ocular hypotension of−2.0 to −2.2 mmHg (p<0.05) the following day. Conclusions: The rebound tonometer waseasy to use and accurately measured IOP in the rhesus monkey eye.

[学科分类] 生物化学/生物物理