Quantification of dolichol in the human lens with different types of cataracts

[摘要] Purpose: To quantify and characterizedolichol species in cataractous and clear human lenses. Methods: Whole lenses were collectedfrom cadaver eyeballs from the C.H. Nagri Eye Bank and Red CrossSociety Eye Bank (Ahmedabad, India). Cataractous nuclei were collectedafter extracapsular cataract extraction (ECCE). Wet weight for all thelenses was taken and were stored at –50 °C until used. Dolicholwasextracted using a standard protocol and then analyzed using HighPerformance Liquid Chromatography (HPLC) on a 4.6 mm×60 mmHypersil-Octadecylsilane (ODS; 3 μm) reversed phase column using aWaters dual pump apparatus, a Waters gradient programmer, and anultraviolet (UV) detector set at 210 nm. Dolichol 13 was used as aninternal standard, and dolichol mixture from the liver was used as anexternal qualitative standard. Results: The highest dolicholconcentration was found in nuclear cataract (2.54±0.6 μg) followed byposterior subcapsular cataract (1.4±0.35 μg), and the lowest levelswere observed in cortical cataract (0.37±0.06 μg). The level ofdolichol concentration in cataractous lenses was statisticallysignificantly higher than the levels in clear lenses (1.0±04.3 μg;p<0.01). Conclusions: The dolichol concentrationwas significantly higher in lenses with nuclear cataract. A significantdifference in dolichol concentration was observed between the differenttypes of cataract. It suggests that dolichol and other isoprenoids maybe associated with cataractogenesis.

[学科分类] 生物化学/生物物理