Monocyte chemoattractant protein (MCP)-1 −2518 A/G SNP in Chinese Han patients with VKH syndrome

[摘要] Purpose: Vogt-Koyanagi-Harada (VKH)syndrome is an autoimmune disease. The monocyte chemoattractantprotein-1 (MCP-1) gene has been implicated in the pathogenesisof certain autoimmune diseases. The aim of this study was to examinewhether a MCP-1 polymorphism was associated with VKH syndrome. Methods: A case-control analysis wasperformed using genomic DNA samples from 307 VKH patients and 319 age-,sex-, and ethnically-matched healthy controls. The MCP-1polymorphism at the −2518 A/G locus was genotyped using polymerasechain reaction restriction fragment length polymorphism (PCR-RFLP)assay. Results: The distribution of genotypicfrequency of the MCP-1 −2518 A/G polymorphism in all subjectsdid not deviate from Hardy–Weinberg equilibrium (HWE; p>0.05).Allelic and genotypic frequency analysis revealed no significantdifference between VKH patients and healthy controls for the MCP-1−2518 A/G polymorphism (p>0.05). No significant differences werefound according to gender and neither was found according toextraocular findings including neck stiffness, tinnitus, alopecia,poliosis, dysacusia, scalp hypersensitivity, and vitiligo. Conclusions: The result suggests thatthe susceptibility to VKH syndrome in Chinese Han patients may be notinfluenced by the MCP-1 −2518 A/G polymorphism.

[学科分类] 生物化学/生物物理