The association of single nucleotide polymorphisms in theMMP-9 genes with susceptibility to acute primary angle closureglaucoma in Taiwanese patients

[摘要] Purpose: To study the relationships between single nucleotidepolymorphisms (SNPs) of extracellular matrix, matrix metalloproteases(MMPs), tissue inhibitors of MMPs, and other glaucoma-associated genesand acute primary angle closure glaucoma (PACG).Methods: We extracted DNA samples from 78 adult patients with acutePACG and 86 control subjects to study the relationships between thesespecific genes and acute PACG. Genotyping was performed for 35 genes bythe GenomeLab SNPstream genotyping system after PCR amplification ofchromosomal DNA. The association between these genetic polymorphisms andrisk of primary PACG was estimated by χ2 and logisticregression.Results: The genotyping success rate was 99%. Genotyping for theMMP9 site (rs2664538) was significantly different between the twogroups (p=0.000001) and the odds ratio was 2.586 (95% CI: 1.715-3.898,p<0.00001). However, there were no associations of SNPs to othergenes in patients with acute PACG.Conclusions: Our results reveal that SNP rs2664538, which is locatedat the MMP9 gene, is likely to be associated with acute PACG.

[学科分类] 生物化学/生物物理