Effect of intraocular surgery and ketamine on aqueous and serumcytokines

[摘要] Purpose: To determine the effect of intraocular surgery andanesthesia on aqueous and serum cytokines.Methods: Patients undergoing routine cataract surgery under generaland local (peribulbar) anesthesia were randomized to those given generalanesthetic with and without the use of ketamine and those having localanesthesia. Aqueous and serum levels of cytokines were collected atcommencement of surgery and were determined by an immunoassay usingmulti-analyte biochip array technology at 18 h post-operatively.Results: At 18 h postoperative, all patients (37) showed significantand many fold increases in their aqueous levels of interleukin(IL)-1α, IL-1β, IL-4, IL-6, IL-8, vascular endothelial growthfactor (VEGF), tumor necrosis factor (TNF)-α, interferon(IFN)-γ, epidermal growth factor (EGF), and monocyte chemotacticprotein (MCP)-1. There was little to no increase in IL-2 and IL-10.Significant increases in some cytokines (EGF, IL-6, and IFN-γ) inthe serum were also found (p=0.038). There were no significantdifferences in aqueous cytokine levels following the use of ketamine orbetween those patients who had general and local anesthesia (0.11

[学科分类] 生物化学/生物物理