Monoclonal Antibody Production against Human Spermatozoal Surface Antigens

[摘要] Introduction: As monoclonal antibodies are potential tools for characterization of soluble or cellular surfaceantigens, use of these proteins has always been considered in infertility and reproduction research. Therefore,in this study, monoclonal antibodies against human sperm surface antigens were produced.Material and Methods: To produce specific clones against human sperm surface antigens, proteins wereextracted using solubilization methods. Balb/c mice were immunized intraperitoneally with the proteinsusing complete Freund’s adjuvant in the first injection and incomplete Adjuvant in the following boosterinjections. Hybridoma cells producing ASA were cloned by limiting dilution.Results: Five stable ASA producing hybridoma clones were achieved and their antibody isotypes weredetermined by ELISA. All the isotypes were of IgG class. Their cross reactivity with rat and micespermatozoa was examined but they did not have any cross reactivity.Conclusion: The produced antibodies can be used in further studies to characterize and evaluate each of theantigens present on human sperm surface and determining their role in fertilization.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] demosponge;spiculite;turbidite;Silesian Basin;Outer Carpathians;Cenomanian