Comparative evaluation of hepatoprotective activity of polyherbalformulations PHF-A and PHF-B in rats

[摘要] The hepatoprotective effects of two polyherbal preparations viz., PHF-A and PHF-B containingconstituents with potential hepatoprotective activity were evaluated using Paracetamol-inducedhepatotoxicity. The treatment effect on lipid peroxidation (LPO) and levels of the associatedantioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase(GPX) were investigated in rats. In Paracetamol-induced hepatotoxicity liver damage wasinduced in Wistar rats by administering Paracetamol (835 mg/kg i. p.) on 7th day. The standarddrug Silymarin (25 mg/kg), PHF-A (3 dose levels i. e. 200, 400 and 600 mg/kg) and PHF-B (3dose levels i. e. 200, 400 and 600 mg/kg) were administered orally to the respective groups oncedaily for 7 days. The levels of marker enzymes (SGOT, SGPT and ALP), and proteins [albumin(Alb), globulin and total proteins (TP)] were assessed in serum. The effects of PHF-A onantioxidant enzymes SOD, CAT, GPX and lipid peroxidation (LPO), were assayed in liverhomogenates to evaluate antioxidant activity. PHF-A (200 mg/kg), PHF-B (200 mg/kg),andsilymarin elicited a significant hepatoprotective activity by lowering the levels of serum markerenzymes and lipid peroxidation and elevating the levels of SOD, CAT, GPX, Alb and TP in adose dependant manner. The present findings suggest that the hepatoprotective effect of PHF-Aand PHF-B in Paracetamol-induced damage might be due to inhibition of Cytochrome P-450activity or prevention of inactivation of antioxidant enzymes.

[学科分类] 药理学