Application of HPLC and HPTLC-Densitometry for theSimultaneous Determination of Camylofin and Diclofenac inPharmaceutical Dosage Form

[摘要] Simple and accurate liquid chromatographic (HPLC) and thin layer chromatographic (HPTLCdensitometry)methods for simultaneous determination of camylofin dihydrochloride (CAM) anddiclofenac potassium (DIC) in tablets were elaborated. The first method was based on isocraticreversed phase liquid chromatography by using RP ODS-C18 column with a mobile phaseconsisting of acetonitrile:25mM potassium dihydrogen phosphate (80:20, v/v) containing 0.1%v/v acetic acid adjusted to pH 7 with triethylamine at a flow rate of 1.5 mL/min and UV detectionat 215 nm. The calibration curve of camylofin and diclofenac was linear in the range of 50-300μg/mL (r>0.999) and 5-30 μg/mL (r>0.999), respectively. The second thin layerchromatographic method employed by using pre-coated silica gel G60–F254 aluminum sheetusing mobile phase chloroform:ethyl acetate:methanol:ammonia (5:3:2:0.1,v/v) and quantitationwas achieved using spectrodensitometrically at 215 nm. The calibration curve of camylofin anddiclofenac was linear in the range of 1500-9000 ng/spot (r>0.999) and 150-900 ng/spot(r>0.999), respectively. The validity of the methods was confirmed using the recovery studies,precision and limit of detection. Both techniques were applied successfully for the analysis ofcamylofin and diclofenac in tablets form. The results obtained from both procedures werestatistically compared using the Student’s-t and F-variance ratio tests.

[学科分类] 药理学