Development and Validation of LC Method for SimultaneousDetermination of Piroxicam and Paracetamol in NewPharmaceutical Formulation

[摘要] In this perspective, we present a reverse phase high performance liquid chromatographicmethod for simultaneous determination of piroxicam and paracetamol in commercialpharmaceutical dosage forms. The resulting contents of tablets were baseline resolved on areverse phase Zorbax SB C18, (250 x 4.6 mm, 5 mm) analytical column. Mobile phase containsmethanol, acetonitrile and 50 mM sodium dihydrogen orthophosphate (27:16:57 v/v) at a flowrate of 1 mL/min. UV detector was set at a wavelength of 254 nm. The resolution betweenparacetamol and piroxicam was less than five. Developed method was extensively proved to berobust for the titled drugs. This method was shown to be linear, correlation coefficient ofparacetamol and piroxicam was 0.9986 and 0.9990 respectively. The percent recovery forparacetamol and piroxicam ranged between 100.5-101.3 and 100.6-100.8 respectively. Thepercent relative standard deviation for six replicates was less than 2 .The limit of detection andlimit of quantification for paracetamol was 140 ng / mL and 400 ng / mL for piroxicam 29 ng /mL and 70 ng / mL . Sample concentrations were measured on weight basis to avoid internalstandard. Proposed method is suitable for quantitative determination of the titled drugs in theircommercial samples of tablet formulation with respect to assay.

[学科分类] 药理学