Simultaneous Determination of Mefenamic Acid and Ethamsylateby Area Under Curve Spectrophotometric Method

[摘要] The method for the simultaneous determination of Mefenamic acid and Ethamsylate byspectroscopy has been developed. The simple, accurate and precise method includes Area Underthe Curve (AUC) method. From a solvent effect studies and the spectral behaviours ofMefenamic acid and Ethamsylate, methanol was selected as solvent. Mefenamic acid showsmaximum absorbance at 337 nm and Ethamsylate shows maximum absorbance at 306 nm. Forthe AUC method, the wavelength ranges between. 337-344 nm and 300-306 nm were selectedwith reference to the absorbance curves plotted between the wavelengths of 200-400 nm. Thismethod allows rapid analysis of two drug combination. The results of analysis were validatedstatistically and by recovery studies by following ICH method validation guideline. Tabletcontaining both drugs was assayed using the methods developed, showing a good accuracy andprecision.

[学科分类] 药理学