Absorbance correction method for the simultaneous estimation of norfloxacinand tinidazole in API and combined tablet dosage formulation by UVspectrophotometry

[摘要] This research focuses on to the estimation of the concentrations of Norfloxacin and Tinidazole simultaneously in API and combined tablet formulation using a very new , simple, accurate and precised method called ‘Absorption correction method’ using UV spectrophotometer. Beer’s law was obeyed for Norfloxacin in the range of 1-7μg/mL at 278nm and also for Tinidazole in the range of 4-247μg/mL at 316nm. From the accuracy, percentage recovery for Norfloxacin and Tinidazole was found to be in the range of 98.33- 101.68% and 98.52-101.44% respectively. %RSD values reported were less than 2. As all the validation parameters were found to be within the limits given by ICH guideline, the developed method was found to be linear, accurate, precised and rugged.

[学科分类] 药理学