A WT1 expressing metastatic human kaposi sarcoma xenograft model

[摘要] We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1^wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.

[学科分类] 生理学与病理学

[关键词] metastasis