Analysis of the expressed immunoglobulin variable region heavy chain gene products in paraproteins from Iranian patients with multiple myeloma

[摘要] The frequency of expression of immunoglobulin (Ig) variable region heavy (V_H) chain gene products was studied in 43 Iranian patients with mutiple myeloma (MM). The expressed V_H gene families and associated cross-reactive idiotypes (CRI) were analysed by immunoblotting and ELISA, using peptide-induced polyclonal antibodies specific for V_H1-V_H6 gene families and monoclonal antibodies (MAb) recognising CRI linked to theV_H1, V_H3, V_H4 and V_H6 gene families. The results revealed that the V_H3 family (60.5%) was the most predominant gene family. In contrast, no paraproteins were encoded by genes from the V_H2 gene family and only 2.3% were encoded by the V_H5 family. The panel of paraproteins tested rarely expressed the probed V_H-associated CRI. Our results suggest that: 1-The Ig V_H genes, may not be randomly expressed in the malignant plasma cells from Iranian patients with MM. 2- Some of the genes seem to be negatively selected or highly mutated, as evidenced by the lack of expression of the probed CRI.

[学科分类] 生理学与病理学

[关键词] cross-reactive idiotype