Effect of cerium oxide nanoparticles to inflammation and oxidative DNA damages in H9c2 cells

[摘要] Occupational heart disease have occurred continuously, making social problems, and increasing the needs for its effective prevention. As natural antioxidants, selenium (Na₂SeO₃) and cerium oxide (CeO₂) nanoparticles, we verified the effect with suspected cardiotoxic chemicals, 1,1,1-trichloroethane (TCEtn) based on the myocardial cell line due to inflammation and oxidative DNA damage, and reliable anti-cardiotoxic effects of selenium and CeO₂ nanoparticles. We measured the changes of gene expression with real-time RT-PCR, and oxidative DNA damage with Fragment Length Analysis with Restriction Enzyme (FLARE) assay in H9c2 cell line, and discuss their molecular mechanism from these data. In the result, it has anticytotoxic effect with CeO₂ nanoparticles below 100 μM which the particles dispersed well. The early expression of COX2 mRNA is increased with TCEtn but decreased with Na₂SeO₃, CeO₂ nanoparticles, has some anti-inflammatory effect. The PPARγ is much increased with all of TCEtn, Na₂SeO₃, CeO₂ nanoparticles in 36 hour pretreat, are evaluated their activation to cytokines, transcription factors related to overcome and decrease the cardiotoxic effects of test chemical. With the oxidative DNA damage, the CeO₂ nanoparticles have more active anti-oxidative effect to selenium.

[学科分类] 分子生物学，细胞生物学和基因

[关键词] Gene expression regulation