Antigenicity of β-Propiolactone-Inactivated Virus Vaccines

[摘要] 1. 1. A simple and rapid procedure for the preparation of inactivated virus vaccine is described using β-propiolactone (BPL), an agent not previously employed in the preparation of vaccines.2. 2. β-propiolactone is capable of inactivating viruses in 10–15 minutes at 37°C whereas formalin and phenol require days for the same degree of inactivation under parallel conditions.3. 3. Satisfactory inactivated vaccines can be prepared with eastern equine encephalomyelitis virus, rabies virus and MM-virus with the use of BPL.4. 4. A significantly higher degree of antigenicity is demonstrated with β-propiolactone-inactivated virus vaccines than with formalin- and phenol-inactivated virus vaccines.5. 5. Two to four times the minimal effective viricidal concentration of BPL can be used in the preparation of virus vaccines without critical loss in antigenicity.

[学科分类] 生物科学（综合）