Abstracts of Papers Presented at the Third International Complement Workshop, Harvard Medical School, Boston, Massachusetts, June 3–5, 1968

[摘要] Target cells consisted of 51Cr-labeled chicken erythrocytes which were treated with rabbit antibody and purified human complement components to produce intermediate complement-cell complexes. They were incubated (in tissue culture medium containing 5% heat-inactivated fetal calf serum chromatographically depleted of C′8 and C′9) for maximally 15 hr with various preparations of human blood leukocytes at a leukocyte-target cell ratio of 50:1. Cell damage was measured by determining the proportion of 51Cr which was released into the medium. The following results were obtained: 1) Cell preparations consisting of 75% lymphoid cells and 25% polymorphonuclear leukocytes (PMN) damaged both C′3- and C′7-target cells, the latter more rapidly and extensively than the former. 2) Purified monocytes exhibited the same cytotoxic effect as the lymphoid cell-PMN mixture. 3) Purified lymphocytes damaged only C′7-target cells but not C′3-target cells. 4) None of the human cell preparations adversely affected target cells which had reacted only with C′1, C′2 and C′4.

[学科分类] 生物科学（综合）