Synthesis and Secretion of Immunoglobulins by Established Cell Lines of Human Hematopoietic Origin

[摘要] The production of immunoglobulins by three established human hematopoietic cell lines has been studied. RPMI no. 8235 produced only κ type IgG, RPMI no. 4666 produced κ type IgA and free κ type light chains indicating an imbalance of heavy and light chain synthesis. These two cell lines are both derived from the buffy coat of patients with chronic myelogeneous leukemia. The third cell line RPMI no. 8226, which is derived from the buffy coat of a patient with multiple myeloma, produced only free λ type light chains very actively without any indication of the production of heavy chains, and appears to be a culture of cells belonging to a malignant plasma cell line.The immunoglobulins produced by these cell lines resemble pathologic immunoglobulins produced in individuals with multiple myeloma. Each immunoglobulin was of a single light chain type and showed a well defined electrophoretic mobility.The time course of immunoglobulin accumulation and cell proliferation indicated that the cells were able to synthesize and secrete immunoglobulins actively only while they were able to proliferate. In their most active phases, the cells of both RPMI nos. 8235 and 4666 synthesized and secreted about 3 µg of immunoglobulins per 105 cells per day, whereas the cells of RPMI no. 8226 produced about 20 µg of light chains per 105 cells per day.

[学科分类] 生物科学（综合）