Antibody-Producing Cells in Rabbits Injected with Soluble BSA

[摘要] A hemolytic plaque technique has been modified for detecting antibody-producing cells in lymphoid tissues of rabbits injected with soluble BSA. A soluble carbodiimide was used to couple BSA to either heterologous (goat) or homologous erythrocytes. In the latter case, background activity was completely eliminated. Optimal concentrations of BSA and coupling agent are described. The effects on plaque production of incubation time and erythrocyte concentration are discussed. Ammonium chloride was used to remove rabbit erythrocytes, which obscured plaque formation, from spleen suspensions. While incubation with complement (direct assay) detected hemolytically efficient antibody, addition of anti-RγG to the complement (indirect assay) was required to develop plaques due to hemolytically inefficient antibody. Antiglobulin produced in some species inhibited rather than enhanced plaque production, and electrophoretic fractionation of these antisera yielded a predominant blocking fraction and an amplifying fraction. Antisera produced in squirrel monkeys provided consistent amplification without fractionation.

[学科分类] 生物科学（综合）