Communications

[摘要] Although it has been reported previously that strain 13 guinea pigs cannot react immunogenically against lysine-containing polymers and polypeptidyl proteins (1–5), reinvestigation of this problem has indicated a major disagreement with these results.Guinea pigs of inbred strain 13 weighing 300 to 400 g were obtained from the closed colony at the National Institutes of Health, Bethesda, Maryland, and from the Rocky Mountain Laboratory, Missoula, Montana. They were immunized with random polymers of α-l-amino acids incorporated in complete Freund's adjuvant. Additional studies employed the polymers of α-d-amino acids aggregated with methylated bovine serum albumin (MBSA). The basic protocol was the same as that employed previously (6, 7). Animals were injected twice, at intervals of 2 weeks, with 100 µg of the polymer plus adjuvant. Three weeks after the second injection the animals were bled from the retroorbital sinus and were skintested with 100 µg of the specific polymer.

[学科分类] 生物科学（综合）