Abstracts of Papers Presented at the Third International Complement Workshop, Harvard Medical School, Boston, Massachusetts, June 3–5, 1968

[摘要] We have presented evidence that the first component of guinea pig complement (C′1gp) is synthesized by columnar epithelial cells of the small intestine. We now report that isolated segments of human colon and to a lesser extent ileum are capable of synthesizing hemolytically active C′1hu. This conclusion is based on the following evidence: After elimination of C′1hu from tissue with EDTA, we found that segments of the intestinal tract in short-term organ culture showed a 100- to 1000-fold increase in C′1hu activity. The rate of production of C′1hu in human intestine was highly temperature dependent; C′1hu production was reversibly inhibited by puromycin and actinomycin D. Furthermore, 14C-labeled amino acids were incorporated into molecules which behaved like C′1. No significant C′1hu synthesis was observed in isolated segments of jejunum, stomach, liver, kidney, lung, spleen or lymph node.

[学科分类] 生物科学（综合）