Iso-Antibodies to a β Globulin that Detects Spontaneous Delayed Iso-Hypersensitivity in Guinea Pigs

[摘要] The level of spontaneous delayed-type iso-hypersensitivity that is directed toward a β globulin iso-antigen (SF) in guinea pigs can be enhanced three- to five-fold by injecting animals with SF+ serum in Freund's adjuvant. This heightened iso-hypersensitivity can be detected in vitro by inhibiting macrophage migration.Circulating antibodies specific for SF were also produced in SF- guinea pigs immunized with SF+ serum in Freund's adjuvant. These antibodies were of the PCA variety in the majority of animals and of the precipitating variety in a small number of animals. In neutralization tests of the PCA antibody using fractions of SF+ serum, SF was found in the β globulin fraction. PCA neutralization was used also to show that the antigens of human and rabbit sera that evoke dermal responses in the guinea pig are different from SF. The precipitating antibodies, used in immunodiffusion, detected a single β-globulin component in SF+ sera but not in SF- sera.The adoptive cutaneous anaphylaxis test (ACA) was used to show that lymph node cells from spontaneously iso-hypersensitive guinea pigs immunized with an unrelated antigen were inhibited from making antibody to the antigen when deposited in the skin of animals possessing SF. The ACA test, thus, will be useful to detect pre-existing iso-hypersensitivity in species that do not readily show delayed dermal reactions.

[学科分类] 生物科学（综合）