Abstracts of Papers Presented at the Third International Complement Workshop, Harvard Medical School, Boston, Massachusetts, June 3–5, 1968

[摘要] A study was undertaken to determine the requirements for lysis of clots formed by the addition of 1 U of thrombin to whole blood diluted 1:10 in phosphate buffer (µ = 0.082, pH 7.4, 4°C). Removal of platelets inhibited normal clot retraction and lysis. Addition of antisera specifically directed against purified γM globulin (19 S cold agglutinin), C′4, C′3, and plasminogen inhibited normal clot retraction and lysis. Antisera to γG globulin and albumin did not inhibit. Washed platelets were preferentially agglutinated by antisera to the above proteins. Photomicrographs of clots formed in the presence of γM antisera had fewer platelets and less platelet aggregation than control samples. These findings suggest that 1) platelets, a γM globulin, C′4, C′3, and plasminogen may participate in a series of reactions leading to lysis of the clot under these conditions; 2) the γM globulin, C′4 and C′3 are preferentially adsorbed onto the platelet surface under the conditions of the assay; and 3) these platelets with their attached proteins in turn are adsorbed onto the fibrin network.

[学科分类] 生物科学（综合）