The X-Y-Z Scheme of Immunocyte Maturation

[摘要] The first serum antibody detected in mice which spontaneously escaped from BSA paralysis was similar in three ways to that first detected in normal mice 8 days after a primary injection of AP-BSA. Each produced the 4-hr and 72-hr mouse PCA reactions, was similar in avidity, and had comparable low levels of antigen-binding capacity.Antibody produced late after spontaneous escape and that produced late in the normal primary response effected only the 4-hr mouse PCA reaction. Such antibody was of avidity intermediate between the 8-day early anti-BSA and hyperimmune anti-BSA.Only 7Sγl possessed anti-BSA activity by radioimmunoelectrophoresis, whether in mice which spontaneously escaped from BSA paralysis or in normal mice following primary or repeated immunization with either AP-BSA or S-BSA. 7Sγ2, γA, and γM were negative by this criterion. The absence of γM antibody to BSA was confirmed by passive hemagglutination and 2-mercaptoethanol studies on unfractionated antisera; and on antisera fractionated by micro-sucrose gradient ultracentrifugation or by ultracentrifugation of preformed 125I-BSA-anti-BSA complexes. The absence of 7Sγ2 anti-BSA was confirmed by the absence of guinea pig PCA reactions with positive mouse antisera to BSA.The results support the notion that the anti-BSA response in mice newly escaped from paralysis is essentially similar to an early primary response.The discussion has served to emphasize that the concept of reversibly paralyzed cells is in accord with many current models of paralysis involving short-term delays rather than extinction of specific immune responsiveness.

[学科分类] 生物科学（综合）