Delayed Hypersensitivity to Myelin Basic Protein in the Passive Transfer of Experimental Allergic Encephalomyelitis

[摘要] Experimental allergic encephalomyelitis was passively transferred in strain 13 guinea pigs with lymph node cells from donors sensitized with either homologous spinal cord or myelin basic protein in Freund's complete adjuvant. Severity of EAE in cell recipients was proportional to the number of cells transferred. Evidence that disease in cell recipients was not due to inadvertent active sensitization was based on: 1) the significant decrease in induction time after cell transfer as compared to length of induction after active challenge; and 2) the almost complete inability of cells to transfer EAE after being heated to 60°C, a temperature which is known to damage cells but which has no effect on the encephalitogenic activity of the emulsion used for active challenge.Delayed-type skin reactivity to myelin basic protein was correlated with severity of EAE in both cell donors and recipients, whereas circulating antibody to the encephalitogen was not detected in the majority of donors or in any recipient. This evidence strengthens the concept of EAE as a manifestation of delayed hypersensitivity to myelin basic protein.

[学科分类] 生物科学（综合）