Studies on the Chemotherapy of Viral Infections

[摘要] Three compounds containing the ketoaldehyde moiety were studied for activity against the PR8 strain of influenza A virus in vitro and in various embryonated egg systems. These compounds were p - ( p - phenylphenacylideneamino)benzoic acid (8949); 4,4′-biphenyldiglyoxylaldehyde, dihydrate (8234); and p -(α-ethoxy- p -phenylphenacylamino)benzoic acid (8318). All were found to be virucidal against the virus in vitro . When these compounds were suspended in 25% heat-inactivated calf serum, the virucidal effect was diminished by approximately 30- to 100-fold. Also, the virucidal effect was reduced by mixing compound with allantoic fluids in vitro or directly in ovo . All compounds were shown to be effective against the PR8 virus in ovo when the compounds and the challenge virus were given by the allantoic route. However, suspending 8234 in 25% calf serum did not impair its activity in intact eggs. Both 8234 and 8318 were studied in de-embryonated eggs using the one-step growth curve technique. A suppression of the hemagglutinins present in the fluids as well as that contained in the allantoic membrane was observed in the treated as compared with the untreated controls when initial treatment with these compounds was begun as early as 0 to 6 hr or as late as 11 to 12 hr postinfection. Thus, these compounds were shown to have both a virucidal and virustatic effect.

[学科分类] 生物科学（综合）